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CASA

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    Overview

    More than 40 years of evolution, the CASA, commonly identified as the Computer Assisted Sperm Analysis, has been developed with several advanced devices. This advanced technology uses a microscope to further capture the accurate image and analysis of the semen present in men.

    The CASA system that is evolved with the modernization with improved technology can naturally witness the various platforms in a shallow specimen chamber where it can hold images of 500 to >2000 sperm, with 50 or 60 frames per second. Apart from this, while obtaining complex or clear extenders for <2 minutes, it stores ≥ 30 frames and provides detailed summary data for each sperm and population. 

    Everything You Need To Know About CASA

    What Is It?

    CASA, also known as Computer Assisted Semen analysis, is referred to as high-end sophisticated electronics imaging systems to witness the sperm. In simple terms, this is nothing but the advanced software program that helps to gauge dozens of individuals’ sperm parameters. 

    This is a fast and accurate catch-all phrase process required for semi-accurate and accurate semen analysis techniques. Also, these tests can remove the inter-observer bias. Also, it analyzes the base of the sperm with the help of tracking the cell movement and image analysis. 

    However, the whole process is performed on the digitizing tablet that is further attached with the microscope. This gives a quick evaluation of the screen of the computer that is attached to the microscope. The whole system provides immediate and objective detailed results of the semen in a complete organized report.

    Furthermore, the system in real-time provides the precise morphological and morphometric analysis of individual spermatozoids. This system can also provide certain specialized tests, including the sperm DNA fragmentation index and sperm buoyancy.

    Why and when is it done?

    A semen analysis is common to test that helps to figure out the fertility problems of the male gender. However, to undergo the semen analysis, a person has to collect their ejaculated semen specimen into a sterile cup with the help of masturbation. A standard CASA will further provide the information on the following parameters:

    • Acidity present in the Fluid (pH value)
    • Volume of Ejaculate
    • Sperm moving Percent commonly known as Motility
    • Concentration of Sperm
    • The appearance of the Sperm OR Morphology
    • Progressive motility (scoring range from poor (1) – best (4)).

    Process

    In the process of CASA, the semen specimen is placed on the visualizing pallet of the microscope, which includes a high-resolution camera. The video camera scans and puts the data in the computer, where it performs the better analysis by the software called CASA. This computerized analysis will provide a vast amount of detailed parameters that can be highly effective for a fertility specialist. More importantly, it produces an accurate and reproducible result for the specialists, making it a lot easier for them. The specialist can perform any analysis by just clicking the computer’s mouse to get the desired results. 

    The other two varieties of sperm measurement process are induced with CASA that includes the following:

    Sperm analysis and Sperm Motility

    In this process, the numbers of sperm moving rapidly in the forward direction with a progressive manner are calculated to evaluate male fertility. This is, however, performed as in semen; there remain varieties of sperm mobility, including no movement, slow movement, and fast movement in a front way. 

    The CASA tracks and finds out the state of individual sperm and identifies which can produce the pregnancy easily. Some other parameters performed with this technique include:

    • Rapid mobility- Identifying the sperm moving with a rate of 25 um/sec or faster than this. 
    • Overall mobility- All the state of the sperm. 
    • Linearity- The percent of sperm, which are moving in a straight line. 
    • Mean Velocities- The speed of the sperm.
    • Progressive mobility- Amount of sperm moving rapidly and in a straight path.
    • Lateral head displacement or aptitude- The distance of back and forth performed by the sperm in the form of wiggling while moving.

    Semen analysis and Sperm Morphology

    In this process, the ability of the man’s fertility is observed. All men’s semen contains sperm with various appearances, such as the normal appearance and abnormal appearance.

    Results 

    The results of CASA performed over the number of men with 358 first pregnancy planners showed that the concentration of spermatozoa with VCL is >25 μm/s. This resulted in the most significant and unique CASA parameter in forecasting the male fertility potential. Thus, these process parameters can provide an exact amount of results in the individual men.

    Conclusion

    Thus the Computer Assisted Semen Analysis is growing globally and can provide more accurate information and is commonly performed in the hospital clinical laboratories. This can further help individuals to understand the amount of male fertility rate.

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