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Intracytoplasmic morphologically selected sperm injection (IMSI)

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    Overview

    Intracytoplasmic morphologically selected sperm injection (IMSI) is a more advanced method of in vitro fertilization (IVF) in which the spermatozoon is selected at a greater magnification before injection. It is possible to assess the fine integrity of the spermatozoon’s nucleus in this manner and to ensure that the spermatozoon is injected with a normal, vacuole-free head.

    Everything You Need To Know About IMSI

    What is It?

    When used in IVF therapy, Intracytoplasmic Morphologically Selected Sperm Injection (IMSI) is a procedure that involves the examination and selection of sperm under a high-magnification digital imaging microscope before microinjection into the egg. When using IMSI, the sperm is picked before the microinjection, which allows for a more timely procedure. It is accomplished by the use of an inverted microscope, which has a magnification power around 6000 times larger than that of the typical reproductive laboratory microscope (400 times), which is used to perform intracytoplasmic sperm injection (ICSI).

    When diagnosing male reproductive issues and predicting fertilization and pregnancy outcomes, the shape of the sperm (morphology) is critical. Studies have indicated that choosing better-shaped sperm improves the success of in vitro fertilization (ICSI). In addition, IMSI has been shown to increase the odds of in-vitro fertilization and may be utilized if you have tried unsuccessfully to conceive via IVF in the past.

    Many advancements have been achieved in Assisted Reproductive Technology (ART), allowing for the development of new and creative approaches for IVF patients, many of which have been designed to raise the success rates of fertility treatments.

    When it is needed?

    Severe male infertility is one of the most prevalent causes of male infertility, accounting for around 10% of all cases. For example, when a man’s sperm count is low, his sperms are too sluggish, or his sperm morphology is aberrant, he has low sperm quality, and he cannot reproduce.

    In the case of IMSI in particular, it is indicated when the morphology of the sperm is changed; a condition is known as teratozoospermia or teratozoospermia, respectively.

    In addition, IMSI may be utilized in the following situations if necessary:

    • Failure of IVF/ICSI procedures
    • Embryos of poor quality that have stopped developing
    • Implantation failure is a serious problem
    • Oligoasthenoteratozoospermia with severe oligoasthenoteratozoospermia
    • DNA fragmentation in the sperm is severe
    • Infertility that has lasted for an extended time without a known cause

    It should be emphasized that IMSI is not routinely used in in vitro fertilization (IVF) laboratories. It is rarely utilized in very rare circumstances due to the exorbitant cost and the fact that it does not raise the success rates in all situations when employed.

    Procedure

    When it comes to IMSI cycles, the same rules apply as when it comes to ICSI cycles. In the laboratory, sperm pellets are first resuspended in a solution of an adequate concentration. In terms of how the sperms are picked, the only difference between IMSI and ICSI is the use of a microscope. MSOME (Motile Sperm Organelle Morphology Examination) technology is used in the construction of this microscope, which is equipped with a digital imaging system. Fertilization of the eggs occurs with a conventional microinjector after the embryologist has selected the best sperms from an ICSI plate.

    Benefits

    Among IMSI’s most significant advantages are its capacity to correctly see sperm cells, which has enabled an increase in spermatozoa of more than 6300-fold. As a result, IMSI offers a 15-fold advantage over standard microscopy regarding IVF and ICSI procedures.

    Real-time selection of live sperm is achievable with the use of IMSI. In addition, it does away with the necessity for potentially dangerous dye tests to determine sperm quality.

    The exquisite detail provided by this examination of the sperm may allow doctors to detect problems in the sperm’s head, neck, and tail. The fragmentation of sperm DNA has been connected to these problems in the past.

    While the IMSI strategy has a beneficial influence on miscarriage rates, it also affects fertility and conception rates.

    Why does IMSI take too many steps?

    It is not recommended because it is a time-consuming procedure that should only be carried out by someone who has received substantial expertise in the subject. When two embryologists collaborate on a process, the operation may be completed more quickly and objectively.

    Furthermore, the use of a high-powered microscope makes it tough to discover and identify individual sperm cells, which makes the situation much more challenging.

    Conclusion

    Individuals and couples who have previously failed IVF cycles should consider IMSI, as well as those who have a male infertility component. Unfortunately, further papers are required to demonstrate the advantages of using IMSI and assess the associated expenses. In addition to the expense of IVF, IMSI is expected to be twice as costly and need additional expert training and equipment, making it impossible for everyone to afford the treatment. IMSI, on the other hand, promises a stride forward in the field of assisted reproduction.

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